Ler livro online, o resumo Methods In Pulmonary Research – Taylor, Aubrey E. – ISBN: 3764354275. Confira preço em loja online confiável.
This volume presents a comprehensive review of methods used to study physiology and the cell biology of the lung. The book covers the entire range of techniques from those that require cell cultures to those using in vivo experimental models. Up-to-date techniques such as intravital microscopy are presented. Yet standard methods such as classical short circuit techniques used to study tracheal transport are fully covered. This book will be extremely useful for all who work in pulmonary research, yet need a practical guide to incorporate other established methods into their research programs. Thus the book will prove to be a valuable resource for cell biologists who wish to use organs in their research programs as well biological scientists who are moving their research programs into more cell related phenomena. Contents: Measurement of lung function in rodents in vivo Application of aerosols Lymphatics Neurogenic inflammation in the airways: Measurement of microvascular leakage Intravital microscopy: airway circulation Intravital microscopy: surface lung vessels The isolated perfused lung Segmental vascular resistance and compliance from vascular occlusion The bronchial circulation Lung explants Tracheal preparations Bronchoalveolar lavage Evaluation of secretory and transport processes which determine the composition of airway surface liquid Isolation of type II alveolar epithelial cells Endothelial cells Cyropreservation of human pulmonary tissues Studying lung ultrastructure Autoradiography in the lung Assessment of surfactant function Appendix 1: Physiological tables, formulas and facts Appendix 2: List of suppliers