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Interest in the study of the biology of aging especially as it relates to humans has increased steadily in the last 50 years and continues today to be at the forefront of experimental biology. Due to the many recent advances in this area selection of the best experimental approach may be difficult. It is the purpose of this laboratory manual to provide the researcher with a specific group of techniques with emphasis on hands-on information. A variety of protocols using molecular, cellular and physiological techniques are presented in detail and appropriate animal models have been selected for the study of aging and aging-related diseases. Non-invasive in vivo techniques are described for the study of Alzheimer’s dementia and cardiovascular diseases. Techniques for detection of oxidative processes are presented to explore the role of free radicals in causing cell damage and mutations as well as techniques to study apoptosis for its role in cell aging.
Autor: Sternberg, Hal